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Explanation of Host Cell Protein (HCP) ELISA and HCP Antibody Coverage Analysis
https://www.cygnustechnologies.com/antibody-affinity-extraction-aaetm
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CHO HCP ELISA Kit, 3G F550-1 from Cygnus Technologies
This new Cygnus Technologies’ CHO HCP ELISA Kit, 3G proves the specificity and sensitivity (LOD ~0.3 ng/ml, LLOQ ~1 ng/ml) to detect HCPs with reproducibility …
Source: www.biocompare.com
Date Published: 1/3/2022
View: 3445
(F550) CHO HCP ELISA Kit, 3G – Cygnus Technologies – CiteAb
Kit [F550] CHO HCP ELISA Kit, 3G by Cygnus Technologies. … Kit Type. ELISA. Target. Chinese Hamster Ovary Host Cell Proteins.
Source: www.citeab.com
Date Published: 12/2/2022
View: 6458
CHO HCP ELISA Kit, 3G | F550-1 – Maxanim
CHO HCP ELISA Kit, 3G. CAT# F550-1. Manufatured by Cygnus Technologies and Supplied by Gentaur Group in UK and Europe.
Source: maxanim.com
Date Published: 8/11/2022
View: 7434
CHO HCP ELISA Kit, 3G *replaced with CHO HCP … – BIOZOL
The “CHO 3G” ELISA is more sensitive than previous kits, able to detect HCPs in the range of 100 parts per billion for a variety of antibodies and other …
Source: www.biozol.de
Date Published: 10/3/2022
View: 4811
CHO HCP ELISA Kit, 3G | F550-1 | Cygnus Technologies
F550-1 | CHO HCP ELISA Kit, 3G | Packing: 1 kit | Cygnus Technologies products available at Bio-Connect.
Source: shop.bio-connect.nl
Date Published: 1/12/2021
View: 8206
Cygnus Technologies CHO HCP ELISA KIT, 3G
Cygnus Technologies CHO HCP ELISA KIT, 3G. SDP. Manufacturer: Cygnus Technologies F5501. Encompass. Catalog No. NC1738021. $965.58 / Each of 1.
Source: www.fishersci.com
Date Published: 5/2/2022
View: 6427
CHO Host Cell Protein ELISA Kit 3rd Generation
The CHO HCP ELISA Kit, 3G proves robust performance supported by decades of in-house technical expertise. The CHO HCP ELISA Kit, 3G is able to…
Source: www.bioprocessonline.com
Date Published: 3/10/2021
View: 3716
CHO HCP ELISA kit, 3G, Mammalian – cogershop.com
CHO HCP ELISA kit, 3G. This CHO HCP 3rd Generation ELISA kit uses a new and even more broadly reactive antibody than our earlier kits. The kit incorporates …
Source: www.cogershop.com
Date Published: 11/14/2022
View: 567
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주제에 대한 기사 평가 cho hcp elisa kit 3g
- Author: Cygnus Technologies
- Views: 조회수 1,832회
- Likes: 좋아요 11개
- Date Published: 2020. 5. 13.
- Video Url link: https://www.youtube.com/watch?v=qNL9oAtbFM0
CHO HCP ELISA Kit, 3G F550-1 from Cygnus Technologies
Description
This CHO HCP 3rd Generation ELISA Kit, Item Number F550-1 will be replacing the current CHO HCP ELISA Kit, 3G, Item Number F550. Cygnus has manufactured, characterized, and qualified large pools of the new reagents that will replace the current reagents. The antibodies were developed and purified in the same way as the original critical reagents in the F550 Kit. The coverage of the new antibodies to the CHO HCP antigen from CHO-S and CHO-K1 strains was determined to be ~86% by Antibody Affinity Extraction which is not significantly different from the current antibodies in the F550 kit. More importantly, Cygnus Technologies has demonstrated the equivalency of the new antibodies (3G-0016-1-AF) in the 3G CHO ELISA (F550-1) using a panel of CHO samples from a number of independent biomanufacturing processes. Similar to the F550 “CHO 3G” ELISA, the F550-1 “CHO 3G” ELISA is more sensitive than previous kits, able to detect HCPs in the range of 100 parts per billion for a variety of antibodies and other therapeutic proteins expressed in CHO. This data indicates the kit is truly a generic method that can likely be validated as not only a process development tool, but in most cases as a lot release test, eliminating the need to develop a custom or process specific assay. This new Cygnus Technologies’ CHO HCP ELISA Kit, 3G provides the specificity and sensitivity (LOD ~0.3 ng/ml, LLOQ ~1 ng/ml) to detect HCPs with reproducibility that supports regulatory compliance.
CHO HCP ELISA Kit, 3G *replaced with CHO HCP ELISA Kit, 3G (F550-1)*
This product has been depleted and replaced with CHO HCP ELISA Kit, 3G (F550-1) Description This CHO HCP 3rd Generation ELISA Kit uses a more broadly reactive antibody than our earlier kits and incorporates a number of other improvements for the detection of CHO HCPs. The “CHO 3G” ELISA is more sensitive than previous kits, able to detect HCPs in the range of 100 parts per billion for a variety of antibodies and other therapeutic proteins expressed in CHO. It has been extensively qualified with a large number of drug substances and in-process samples. This data indicates the kit is truly a generic method that can likely be validated as not only a process development tool, but in most cases as a lot release test, eliminating the need to develop a custom or process specific assay. The antibody has been evaluated for reactivity to individual HCPs using the conventional 2D Western Blot as well as a method we have shown to be superior to 2D WB, which we call AAE, Antibody Affinity Extraction. AAE demonstrated the antibody is reactive to more than 1000 individual HCPs from conditioned media and cell lysates in both a CHO-S strain as well as a K1 strain. These 1000+ HCPs represent more than 98% of the total mass of protein as indicated by protein detection methods orthogonal to ELISA. As such, this antibody may overcome concerns about missing any significant individual HCPs. This ELISA is also more robust than the earlier kits showing less sample matrix interference and improved dilution linearity. If you have found the F015 and CM015 kits lacking in any way meeting your HCP detection needs, or if you are considering the need for a process-specific assay, we recommend you evaluate this 3rd Generation CHO HCP ELISA kit.
Cygnus Technologies CHO HCP ELISA KIT, 3G
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CHO Host Cell Protein ELISA Kit, 3rd Generation
The CHO HCP ELISA Kit, 3G provides robust performance supported by decades of in-house technical expertise. The CHO HCP ELISA Kit, 3G is able to detect HCPs in the range of 100 parts per billion for a variety of antibodies and other therapeutic proteins expressed in CHO. It has been extensively qualified with a large number of drug substances and in-process samples. This data indicates the kit is truly a generic method that can likely be validated as not only a process development tool, but in most cases as a lot release test, eliminating the need to develop a custom or process specific assay. The antibody has been evaluated for reactivity to individual HCPs using the conventional 2D Western Blot as well as a method we have shown to be superior to 2D WB, which we call AAE, Antibody Affinity Extraction. AAE demonstrated the antibody is reactive to more than 1000 individual HCPs from conditioned media and cell lysates in both a CHO-S strain as well as a K1 strain. These 1000+ HCPs represent more than 98% of the total mass of protein as indicated by protein detection methods orthogonal to ELISA. As such, this antibody may overcome concerns about missing any significant individual HCPs.
CHO HCP ELISA kit, 3G, Mammalian
CHO HCP ELISA kit, 3G This CHO HCP 3rd Generation ELISA kit uses a new and even more broadly reactive antibody than our earlier kits. The kit incorporates a number of other improvements in the detection of CHO HCPs. This new ELISA is more sensitive than the previous kits, able to detect HCPs in the range of 100 parts per billion for a variety of antibodies and other therapeutic proteins expressed in CHO. It has been extensively qualified with a large number of drug substances and in-process samples. This data indicates the kit is truly a generic method that can likely be validated as not only a process development tool, but in most cases as a lot release test, eliminating the need to develop a custom or process specific assay. The antibody has been evaluated for reactivity to individual HCPs using the conventional 2D Western Blot as well as a method we have shown to be superior to 2D WB, which we call 2D HPLC-ELISA. This 2D HPLC-ELISA method demonstrated the antibody is reactive to more than 750 individual HCPs from conditioned media and cell lysates in both a CHO-S strain as well as a K1 strain. These 750+ HCPs represent more than 98% of the total mass of protein as indicated by protein detection methods orthogonal to ELISA. As such, this antibody may overcome concerns about missing any significant individual HCPs.
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