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The VICTOR® Nivo™ provides excellent performance while being the industry’s smallest multimode plate reader – making it a great choice for multi-user labs that need a versatile workhorse instrument. Every VICTOR Nivo comes rainbow-ready, giving you the flexibility of a monochromator, with the high sensitivity of a filter-based instrument.
Key Features:
• Smallest and most affordable multimode plate reader in the industry.
• Certified for use with HTRF® technology.
• Best for research labs with multi-users and low-throughput assays.
•Stacker option to deliver walk away automation, batch processing of up to 20 microplates
•Rainbow Filter Technology: Every VICTOR Nivo is equipped with the ability to hold up to 32 filters spanning the UV-Visible light spectrum. This gives you the flexibility of a monochromator, with the high sensitivity of a filter-based plate reader. All of the wavelengths you need for virtually any application are ready and available at any time. Learn more here: https://bit.ly/2HmuvzK
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victor nivo™ multimode plate reader
The VICTOR Nivo™ Multimode Plate Reader can be used to quantitatively measure light emitting or absorbing substances both from top and bottom …
Source: clsep.zju.edu.cn
Date Published: 11/14/2021
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- Author: PerkinElmer, Inc.
- Views: 조회수 1,556회
- Likes: 좋아요 14개
- Date Published: 2021. 2. 24.
- Video Url link: https://www.youtube.com/watch?v=ElJ47rW5Ndg
How do I choose a plate reader?
Primary considerations when choosing a microplate reader are throughput and flexibility. In labs where throughput is a top priority, a device that can screen 96- to 1536-well microplates in under one minute is worthwhile.
How fluorescence is measured in a fluorescence plate reader?
An Introduction to Plate Readers
Luminescence and fluorescence signals are detected and quantified using luminometers and fluorometers, respectively, and colorimetric and absorbance measurements are detected by light absorption at specific wavelengths.
What does a microplate reader measure?
A microplate reader is a laboratory instrument that is used to measure chemical, biological or physical reactions, properties, and analytes within the well of a microplate. A microplate consists of small wells in which separated reactions take place.
What is multimode plate reader?
A microplate reader that can detect two or more applications is considered a multi-mode plate reader. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP).
Can you use clear plates for fluorescence?
Clear plates are routinely used for fluorescence-based assays, but opaque-walled microplates reduce bleed-through between wells.
Why do we use black plate for fluorescence?
For fluorescent assays, black plates absorb light and reduce background and crosstalk.
How do fluorescence plate readers work?
The emission system of the plate reader uses polarizing filters to analyze the polarity of the emitted light. A low level of polarization indicates that small fluorescent molecules move freely in the sample. A high level of polarization indicates that fluorescent is attached to a larger molecular complex.
What is fluorescent plate reader?
Spectrofluorometers or plate readers with fluorescence detection typically offer greater sensitivity and a wider dynamic range compared to absorbance detection. Because many reagents can be fluorescently labeled, spectrofluorometers are used to detect many biological and chemical processes.
What is the difference between microplate reader and spectrophotometer?
Compared to a standard spectrophotometer that measures one sample at a time in a cuvette, an absorbance microplate reader can measure the same application with higher throughput. It can measure a full 96 well microplate in seconds, or 384 or 1536 well plates in a few minutes.
What is the purpose of a plate reader?
Automated license plate readers (ALPRs) capture computer-readable images of license plates. These high-tech devices allow law enforcement agencies to compare plate numbers against those of stolen cars or cars driven by people suspected of being involved in criminal or terrorist activities.
How does a plate reader measure absorbance?
The sample is typically placed in a cuvette through which light is sent horizontally. An absorbance plate reader offers higher throughput and can measure the absorbance of samples in microplates (typically 96-well or even 384-well) by sending light through each well vertically.
How much does a BioTek plate reader cost?
| Catalog Number | Mfr. No. | Price |
|---|---|---|
| Catalog Number | Mfr. No. | Price |
| BTS1LFA View Documents View Product Certificates | Agilent BioTek S1LFASN | Each for $30,800.00 |
| BTS1LFTA View Documents View Product Certificates | Agilent BioTek S1LFTASN | Each for $33,450.00 |
Is a plate reader a spectrophotometer?
Microplate Readers, or Plate Readers, are a type of spectrophotometer. Both use either a monochromator system or filter-based optics and these lab instruments both collect useful measurements across a variety of methods.
What are microtiter plates used for?
Microtiterplates are convenient, high-throughput tools for organizing tissue culture, PCR tests (such as HIV screening), or immunological assays such as ELISA, RIA, and FIA.. Other applications for PCR plates include analysis of enzyme reactions and compound identification by colorimetric or spectroscopic analysis.
How do plate readers differ from spectrophotometers?
A microplate reader is essentially a spectrophotometer but with a few key differences. While a spectrophotometer works with a monochromator or filter-based optics to measure events in samples just like a microtiter plate reader, a spectrophotometer works with a single small sample size.
What is the difference between microplate reader and spectrophotometer?
Compared to a standard spectrophotometer that measures one sample at a time in a cuvette, an absorbance microplate reader can measure the same application with higher throughput. It can measure a full 96 well microplate in seconds, or 384 or 1536 well plates in a few minutes.
Do license plate covers work Mythbusters?
A speed camera cannot see through a lenticular lens plate cover. While designed to distort the plate from an angle, all or most of the letters of the plate were still identifiable in the tests. Also, the legality of such a device was questioned.
What is the purpose of a plate reader?
Automated license plate readers (ALPRs) capture computer-readable images of license plates. These high-tech devices allow law enforcement agencies to compare plate numbers against those of stolen cars or cars driven by people suspected of being involved in criminal or terrorist activities.
Choosing the Right Microplate Reader
For quick assessment of enzyme activity or protein and nucleic acid quantification, microplate readers have become a reliable and obvious choice for any lab. These multipurpose instruments facilitate research by minimizing operational time, improving processes and efficiency, and saving costs. Microplate readers are everywhere, in drug discovery, basic research, bioassay validation, and biopharmaceutical manufacturing, with applications ranging from simple ELISAs to high-throughput detection.
With a constantly evolving number of reagents, kits, and accessories, determining the right microplate reader for your lab is a challenge. Readers can be equipped with specific or multiple detection modes, accept a variety of plate layouts, and include additional automated functions, with base readers starting at around $4,000 and going up from there. Aligning the right features with current and future lab needs can help determine the best microplate reader for you.
What to consider
Primary considerations when choosing a microplate reader are throughput and flexibility. In labs where throughput is a top priority, a device that can screen 96- to 1536-well microplates in under one minute is worthwhile. If flexibility becomes important in a growing lab supporting a variety of projects, the decision might come down to whether a multimode reader that offers additional capabilities better suits the lab’s needs. Additionally, user experience and benefits such as ease of use and optimization functions, as well as integrated software and built-ins, might offset extra costs.
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With a goal of performing high volumes of biochemical or pharmacological tests and achieving maximum efficiency, high-throughput screening or sample testing must be fast, minimize sample use, and maintain a high level of sensitivity. Microplate readers can play an influential role in the success of an experiment, enabling more difficult to detect targets to be quickly identified in a reproducible manner. Evaluating throughput requirements can help direct plate format and device decisions, such as whether a monochromator, hybrid, or laser detector is most useful.
Another important consideration lies in the assay types to be run and the flexibility of the instrument. Will you be running simple quantitation assays or are there plans for experiments with multiple type requirements, as there are for core facilities? Assay types dictate what modes are needed. Modalities such as absorbance, fluorescence, and luminescence are the most frequently used in laboratories worldwide. Yet, depending on the application, advanced modes including fluorescence polarization or time-resolved fluorescence could be preferred. If there are multiple assay types to be run, flexibility is key. Multimode instruments provide the flexibility to run a variety of applications on the same instrument.
When taking into account user experience and additional features, make sure to focus on current needs and potential growth. Easy-to-use instruments contain more automation, such as wavelength selection, optimization, and built-in protocols with integrated software. More complex workflows and high-throughput assays might benefit from a more comprehensive workstation setup. The latest iterations of readers tend to have modular configurations, where base instruments can be customized to fit a variety of user levels and with upgradable options.
Microplate reader modalities
Microplate readers use different modes to detect light emitted by samples in a plate. Light emission is the result of a biological, chemical, biochemical, or physical reaction, which is then measured and quantified by a detector, usually a photomultiplier tube (PMT).
The type of wavelength selection system can impact what modes can be incorporated as well as what reagent technologies can be used. While filter-based detection is more sensitive and less expensive, instruments with monochromators are more convenient, easy to use, and can run spectral scans across varying wavelengths, expanding the number of assays that can be performed. Newer versions offer hybrid optical systems integrated into one instrument. While hybrids were initially believed to compromise the quality of both approaches, advances in technology have eliminated this concern.
The traditional absorbance microplate reader calculates absorbance as the ratio of light intensity illuminated on and then transmitted through a sample. The amount of absorbed light correlates to the concentration of the sample, so is ideal for nucleic acid and protein quantification or colorimetric reactions. Inclusion of a UV/vis spectrometer enables high-performance and full-spectrum absorbance measurements, instead of requiring specific wavelength selection. This allows faster data collection of typically 1 second or less per well of the entire absorbance spectrum (~200 to 1000 nm).
Fluorescence microplate readers offer sensitive and precise detection of any fluorescence intensity assay, with better dynamic range than absorbance approaches. When a fluorescent molecule emits light upon excitation by a higher energy light source, the emission is filtered, collected, and measured. With a diverse array of fluorophores, fluorescence intensity assays can include single or multiple colors in one experiment. FRET assays can also be measured, where an excited donor transfers energy to the acceptor, which will emit light without external excitation sources. Compatible applications include receptor studies, fusion assays, protease assays, or immunoassays.
Luminescence microplate readers do not need an excitation light source. Instead, light is emitted resulting from a chemical or biochemical reaction. Reactions can be flash (bright and fast) or glow (slow and dim) and can use BRET (bioluminescence resonance energy transfer) as a dual reporter detection assay. High sensitivity, low-noise photon-counting photomultiplier tubes are a popular choice for these devices, along with injectors with variable volume and automated washing functions. Luminescence detection can be used for luciferase reporter assays, cell viability, ATP measurement, and cytotoxicity assays. While wavelength selection is not required, the ability to select multiple wavelengths offers additional benefits for multiplex assays and optimizing signal to noise.
For labs that have a variety of different assays to run, multimode microplate readers might be the best bet. While multimode readers are more expensive than single-mode instruments, there has been a change in approach to lower their cost. These readers can be equipped with a modular configuration that enables selection of those detection modes to suit your current needs and the option to add others in the future as needs change. Standard multimode readers measure absorbance, fluorescence, and luminescence. Iterations of these permit add-ons of additional approaches, including time-resolved fluorescence (includes TR-FRET, HTRF®) and fluorescence polarization and can be further expanded to include AlphaScreen, BRET, dual luciferase reporter assays with injectors, and western blot detection.
Advances and options
With a constant evolution of technology, improvements, enhancements, and additional functions are changing the way we use microplate readers. Recent developments are increasing the speed and sensitivity of assays, in addition to introducing automation for easier operation and upgradable functions that incorporate more of the workflow.
Some multimode readers require manual adjustment of PMT gain voltages to optimize sample signals, which is cost-effective but takes time that can result in loss of sensitivity or signal over-saturation. Other readers have automated the dynamic range selection for easier and quicker assay setup and optimal reading range based on signal intensities, allowing both bright and dim samples to be read with equal sensitivity. Vendors are also integrating excitation lasers to significantly improve performance and lower limits of detection. Compared to xenon lamps, lasers produce higher excitation energy at a specific wavelength for high-throughput assays, TRF, or Alphascreen applications.
Taking a more comprehensive approach, workstations combine multistep workflows into one automated instrument. Microplate assays, such as ELISAs, consist of several biochemical steps including reagent dispensing, incubation, shaking, wash steps, and reading. Automated stackers, heaters, and stirrers for reagents, microplate shakers, and washers, and enhanced touchscreen workflows that simplify tasks and store protocols are making assays ever more convenient.
Look for instruments that can be upgraded with different modalities and accessories should your needs change. A barcode reader, programmable injectors, and dual-channel configurations are useful features that can increase throughput. Additional accessories help to enhance the instrument performance and improve sample processing, from atmospheric control units for live cell-based assays and flexible temperature control to data syncing on network computers and LIMS integration. No matter how simple or complex your reader requirements may be, there are myriad options for both specific needs and broader possibilities.
Absorbance Detection
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Microplate Reader – Plate Reader
Basic information about microplate readers
What is a Microplate Reader?
A microplate reader is a laboratory instrument that is used to measure chemical, biological or physical reactions, properties, and analytes within the well of a microplate. A microplate consists of small wells in which separated reactions take place. These reactions convert the presence of an analyte or the progression of biochemical processes into optical signals. The microplate reader detects these signals and thus quantifies the parameter of interest.
Scientists in the life sciences and pharmaceutical industries strive to improve routine laboratory processes and efficiency by using products or instruments able to save time. A microplate reader can handle up to 3456 samples in minutes or even seconds. A plate reader helps to minimize operational time and to save reagent costs, allowing researchers to dedicate more time to data analysis and the generation of actionable insights.
What is a microplate reader used for?
A microplate reader is used for the quantification of several biological and chemical assays in a microplate. Nowadays, the availability of a plethora of reagent kits enables the exploitation of a microplate reader in different fields and for many different applications. Besides biological, biochemical, and pharmaceutical research, both in academic and industrial environments, plate readers are also used in environmental research, and in the food or cosmetics industry.
Working principle of a microplate reader
A microplate reader detects light signals produced by samples that have been pipetted into a microplate. The optical properties of these samples are the result of a biological, chemical, biochemical, or physical reaction. Different analytic reactions result in different optical changes used for analysis. Absorbance, fluorescence intensity, and luminescence are the most popular and most frequently used detection modes in laboratories worldwide. Additionally, advanced modes such as fluorescence polarization, time-resolved fluorescence, and AlphaScreen® are also available.
Microplate-based measurements detect light signals produced by a sample, converted by a sample, or transmitted through a sample. The signal is measured by a detector, usually a photomultiplier tube (PMT). PMTs convert photons into electricity that is then quantified by the microplate reader. The output of this process is numbered by which a sample is quantified.
Depending on the nature of the optical signal changes during a reaction and consequently on the detection mode, samples may need to be excited by light at specific wavelengths. This light is usually provided by a broadband xenon flash lamp. In order to allow excitation of the sample only by specific wavelengths, the light produced by the lamp is selected by a specific excitation filter or monochromator. To increase sensitivity and specificity, filters or monochromators are equally employed on the emission/detection side. These are usually placed between the sample and the detector.
Multi-Mode Plate Readers
What is a multi-mode plate reader?
A microplate reader that can detect two or more applications is considered a multi-mode plate reader. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP).
Other technologies such as imaging, alsphascreen or western blot detection can be added to some multi-mode plate readers. Consider a system that has the capability to upgrade if current budget is a factor.
PerkinElmer Victor Nivo Microplate Readers
Your login session will expire after 30 seconds and you will lose unsaved information Do you want to extend session? extend your session [ 30 ] Equipment Spectrophotometers Plate readers PerkinElmer Victor Nivo Microplate Readers PerkinElmer Victor Nivo Microplate Readers Description VIDEO
Luminescence
Absorbance UV-visible (260 – 280 nm ; 340 – 1000 nm)
Fluorescence over and under (340 – 850 nm)
Time-resolved fluorescence (TRF/HTRF, TR-FRET, DELFIA, LANCE)
Fluorescence polarization (400 – 850 nm)
Suitable for all plate sizes from 6 to 1536 wells With top and bottom reading : end point, kinetics and well scanning mode
Shaker with three modes : linear, orbital, and double orbital
Temperature control up to 65 °C
Control via intuitive software with predefined programs (requires a computer or tablet)
In addition, MyAssays Desktop Standard software is provided for data analysis
Connectivity : can be operated via WiFi or a network connection, compatible with any operating system
L x D x H : 50 x 15 x 27 cm
Weight : 15 kg
Available options : Dual injectors to monitor in real time the effects of adding a reagent
CO₂ and O₂ control to work on living cells or in hypoxic conditions
Tablet or computer
32 filtres interchangeables
Le Victor Nivo can also be optionally equipped with Alpha laser technology Equipped with all major detection technologies depending on the model :Available options : Source : PerkinElmer, Inc. Readers Filters Accessories No item has been found in DD Biolab catalog, or in your market conditions No item has been found in DD Biolab catalog, or in your market conditions No item has been found in DD Biolab catalog, or in your market conditions You may be also interested in these products AMR-100 Microplate Reader
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FluoroNunc Plates-strips
Strip plate modules
Greiner Bio-One UV-Star plates
Screenstar 96, 384 and 1536 well plates
Plates 96-Wells optical bottom
One Touch™ tips
Graduated tips ClearLine® on hinged lid rack
PerkinElmer VICTOR Nivo
Certified for use with HTRF®, Alpha, LANCE® or DELFIA®
The VICTOR® Nivo™ system packs all popular detection modes into the industry’s smallest multimode reader footprint. The perfect microplate reader for everyday biochemical and cell-based assays, it measures at any wavelength, with top and bottom reading of all technologies. Its software interface is easy to learn and use, and lets you control the instrument from almost any device – perfect for multiuser environments. Enhanced Security software option is available and it includes tools to facilitate 21 CFR Part 11 compliance for integration in regulated environments (GxP).
Victor Nivo Multimode Plate Reader
The Victor Nivo Multi-Mode microplate reader is an advanced platereader that is capable of Fluorescence, Luminescence, UV-Visible Absorbance, FRET, and BRET detection modes. It reads 6- to 384- well microplate formats
Our Victor Nivo Multi-Mode Plate Reader does not have an injection system.
Compatible with multi-well plates: 6 well to 384 well
Fluorescent Wavelength according to available filters: Filters (for both excitation and emission): 645/75, 460/30, 540/10, 460/80, 355/40, 320/75, 405/10, 480/30, 530/30, 595/10, 615/8, 665/8, 560/10, 580/10, Unfiltered Lum, 700 nm IR blocker
Absorbance Wavelength range 200-999 in 1 nm increment.
Luminescence Wavelength range 300-700 nm (top detection)
Plate shaker, temperature control and time lapse reads
Plate Types: (Please use flat bottoms)
Absorbance readings: clear plates with clear bottom
Fluorescence readings: black plates with clear bottom
Luminescence readings: white plates with white bottom
Plate shaker, temperature control and time lapse reads
** Note: If you are not a Life Sciences major then you will not have immediate access to the computer. Please email the technician with your netID at [email protected] and they will have IT add you as a user on the computer.
VICTOR NIVO USER MANUAL
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