당신은 주제를 찾고 있습니까 “doric fiber photometry – doric hybrid cannula“? 다음 카테고리의 웹사이트 https://ro.taphoamini.com 에서 귀하의 모든 질문에 답변해 드립니다: https://ro.taphoamini.com/wiki/. 바로 아래에서 답을 찾을 수 있습니다. 작성자 DoricOG 이(가) 작성한 기사에는 조회수 1,208회 및 좋아요 1개 개의 좋아요가 있습니다.
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doric fiber photometry 주제에 대한 동영상 보기
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d여기에서 doric hybrid cannula – doric fiber photometry 주제에 대한 세부정보를 참조하세요
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Does anyone have experience in using Doric Neuroscience …
Fiber photometry is a sensitive and easy way to detect changes in fluorescent signals. The combination of fiber photometry with various fluorescent biomarkers …
Source: www.researchgate.net
Date Published: 9/3/2021
View: 434
Doric社製 ファイバーフォトメトリー – ｜optogenetics.jp
Doric Fiber Photometry System. Doric Lenses社製のファイバーフォトメトリー関連製品です。 ファイバーフォトメトリーの核となる蛍光ミニキューブから、システム全体 …
Source: www.optogenetics.jp
Date Published: 5/15/2021
View: 7186
pyPhotometry – New fluorescent detectors avalable at Doric
I am assembling a fiber-photometry setup to perform two-color recordings. … The output voltage range of the Doric Fluorescence detector is -5V to +5V, …
Source: groups.google.com
Date Published: 3/2/2022
View: 5169
A flexible and versatile system for multicolor fiber photometry …
Here, we propose a novel approach for fiber photometry recordings, … mirrors and mainly assembled from components produced by Doric Lenses …
Source: www.biorxiv.org
Date Published: 3/26/2021
View: 258
Fiber Photometry
Doric Neuroscience Studio Software. – Fiber Photometry Rack to mount the whole system. – All required electrical cables and optical patch cords.
Source: se04c37c463eadeca.jimcontent.com
Date Published: 10/6/2022
View: 2620
Optical fiber interleaver – Doric Lenses – DirectIndustry
For 4 to 19 brain targets, we recommend using the Doric Bundle-imaging Fiber-photometry system where the signals from each fiber within the bundle are …
Source: www.directindustry.com
Date Published: 4/10/2022
View: 6549
Doric Lenses Inc. | Photonics Buyers’ Guide
Assembles laser diode collimating lenses and fiber-co. … Doric Lenses Inc. … cannulas, miniature fluorescence microscopes, and fiber photometry systems.
Source: www.photonics.com
Date Published: 4/8/2021
View: 7663
주제와 관련된 이미지 doric fiber photometry
주제와 관련된 더 많은 사진을 참조하십시오 doric hybrid cannula. 댓글에서 더 많은 관련 이미지를 보거나 필요한 경우 더 많은 관련 기사를 볼 수 있습니다.
주제에 대한 기사 평가 doric fiber photometry
- Author: DoricOG
- Views: 조회수 1,208회
- Likes: 좋아요 1개
- Date Published: 2011. 5. 24.
- Video Url link: https://www.youtube.com/watch?v=GW-7HRGWSqY
Doric社製 ファイバーフォトメトリー
Doric ファイバーフォトメトリー システム
お客様のアプリケーションに合わせたシステムを構成するためのヒントを載せております。
Doric Fiber Photometry System
Doric Lenses社製のファイバーフォトメトリー関連製品です。
ファイバーフォトメトリーの核となる蛍光ミニキューブから、システム全体を制御することのできるコンソールまで、ファイバーフォトメトリーに必要なすべての機器を用意しております。
ファイバーフォトメトリーとは、動物脳内にインプラントされた光ファイバーカニューラを通して、蛍光カルシウムマーカーでタグ付けされたニューロンに励起光を送り、その蛍光を収集し解析する方法です。ヘッドマウント蛍光顕微鏡ではタグ付けされた各ニューロンの活動を観察できますが、ファイバーフォトメトリーでは捉えられたニューロンの蛍光をすべて合わせたものがデータとして測定されます。
ファイバーフォトメトリーでは非常に弱い蛍光の変化を比較的高いノイズレベルから区別して測定する必要があります。
そこでは光源から検出器まで、システムを構成するコンポーネントを慎重に選択する必要があります。
励起光源としてのコネクタ出力LED光源(CLED)は、スペックルフリーの安定した出力と十分なスペクトル強度と多くの波長選択肢を提供します。
Ce:YAG+光源はUVまたは青色のLED光源と必要な波長にフィルタリングされたCe:YAG光源を１つの筐体に収めた光源です。500-600nmの波長域でLED光源よりも大きな出力を必要とされる場合、またはコア径の小さいカニューラを使用する場合にお勧めします。
ファイバーフォトメトリーシステムの心臓部は、蛍光ミニキューブ（FMC）です。光ファイバーカニューラとそれに接続された光ファイバーパッチコードによりサンプルに励起光が照射され、蛍光は収集されて蛍光ミニキューブ（FMC）に戻されます。蛍光ミニキューブ内で分離された蛍光は、高感度のフォトディテクターで検出されます。
蛍光ミニキューブのオプトメカニカル設計、フィルタ選択およびカップリング光学系とそのアライメント精度は、十分なSN比を確保するために非常に重要で、Doric社では高性能な蛍光ミニキューブを提供しています。
一般的には、励起光はmW程度以下の出力が用いられ、測定される蛍光はnW程度です。このような極低レベルのシグナル検出には、低ノイズの光検出器が必要です。このレベルの測定には外部からの光ノイズをできる限り絶縁することが不可欠です。光ファイバーパッチコードの被覆は光遮蔽性があるものを使用します。DCノイズは、プローブまたは光ファイバー自体の自己蛍光から発生する可能性がありますので、このノイズを抑えるために、低自蛍光タイプの光ファイバーを使用しています。またその長さを最小限に抑える必要があります。
pyPhotometry – New fluorescent detectors avalable at Doric
Hi Felix and Aza,
To understand what is going on in your data it is useful to think about the multiple possible sources that may be generating the fluorescent signal. These are:
1. Fluorescence from the flurophores you are expressing in the tissue (GCaMP and mCherry).
2. Autofluorescence from normal brain tissue.
3. Autuofluorescence from the optic fiber between the minicube and the sample.
Our experience is that the 465nm LED excites substantial autofluorescence from the optic fiber in both the green and the red channels, whereas the 560nm LED excites minimal autofluorescence from the optic fiber. You can test this by pointing the optic fiber that normally goes to the subject at a black light absorbent target and varying the power of the 465 and 560nm LEDs. It is worth doing this in both continous mode and time division mode to get a good understanding of what is going on. On our setups, varying the 465nm LED power in continous acquisition mode causes large signal changes for both the green and red channels, due to autofluorscence excited from the fiber. Varying the 465nm LED power in time division mode causes changes in the signal level for the green channel, but not for the red channel, because the red channel samples are being read when the 465nm LED is off. In either mode we see little variation in either channel when we change the 560nm LED power because this led does not excite much autofluorescence from the fiber. Of course, if the fiber is pointed at a fluorescent sample which absorbs at 560nm and emits in the red channel (e.g. mCherry or TdTomato), changes in the 560nm LED power will drive large signal changes in the red chanel.
Given that you see essentially zero signal in the red channel when you are in time division mode, this suggests that there is minimal mCherry expression within range of the fiber (I assume you have the 460nm LED on max power – what light power do you have for each color at the fiber tip). Your observations about how the signal changes when you vary the LEDs powers could be consistent with your signal primarily being autofluorescence from the fibers. It is really hard to know what is causing those large slow shifts you see in the green channel – they do not look very physiological though I don’t know what neuronal population you are trying to record from. They could be movement artifacts or due to the rotary joint if you are using one, though you would normally not expect ~20% signal changes. Have you done any histology yet to verify your fiber placements and virus experssion?
best,
Thomas
Optical fiber interleaver
DESCRIPTION High-Density Fiber-optic Cannula Array features implantable fibers in a hexagonal pattern of 19 holes with 465 µm pitch. The custom optical fiber array patterns that fit selected brain targets are built by placing fibers in appropriate holes. The optical fiber protrusion of each fiber within the array is factory set to the desired length. The insertion of the optical fibers into the brain tissue is facilitated if its tips are angled or conical. The back of the cannula body is made in a form of a receptacle with an orientation guiding pin. High-Density Fiber-optic Cannula Array allows for up to 19 separate optical fiber connections for optical recording and/or stimulation. High Density Fiber-optic Cannula ArrayHigh Density Fiber-optic Cannula Array Matching fiber-optic patch cord with a multiple fiber connector facing the receptacle of fiber-optic cannula array is made with the identical hexagonal pattern and can be securely fastened to the cannula array with a threaded barrel. The configuration of the other end of the fiber-optic patch cord depends on the type of optical recording and/or stimulation system. For 2 or 3 brain targets, an equal number of single-site optogenetics and/or fiber photometry systems can be used in conjunction with this array. For 4 to 19 brain targets, we recommend using the Doric Bundle-imaging Fiber-photometry system where the signals from each fiber within the bundle are re-imaged on a single CMOS or sCMOS chip. SPECIFICATIONS Transmission : > 75% *tested with 200 µm core – NA0.22 optical fibres Optical Fiber 100 or 200 μm core NA 0.66 Protrusion Length Tolerance : +/- 0.1 mm Base Dimension 7.0 x 5.8 mm Mass : 380 mg
Doric Lenses Inc.
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키워드에 대한 정보 doric fiber photometry
다음은 Bing에서 doric fiber photometry 주제에 대한 검색 결과입니다. 필요한 경우 더 읽을 수 있습니다.
이 기사는 인터넷의 다양한 출처에서 편집되었습니다. 이 기사가 유용했기를 바랍니다. 이 기사가 유용하다고 생각되면 공유하십시오. 매우 감사합니다!
사람들이 주제에 대해 자주 검색하는 키워드 doric hybrid cannula
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주제에 대한 기사를 시청해 주셔서 감사합니다 doric hybrid cannula | doric fiber photometry, 이 기사가 유용하다고 생각되면 공유하십시오, 매우 감사합니다.
